The performance of the antennas, which plays an important role in millimeter-wave astronomy and space communication, is often limited by their reflector surface accuracy. Microwave holography is a fast and effective technique for measuring the surface profile of reflector antenna. In this paper, the antenna aperture phase profile is obtained by microwave holography to estimate the deviation between the reflector and the ideal paraboloid of the Tianma 65 m radio telescope. The panels of the Tianma 65 m radio telescope is in radial pattern with 14 rings. Each corner of the panel is fixed on the screw of the actuator to move up and down, and the adjacent corners of the four panels share an actuator. We use the method of plane fitting to calculate the adjustment value of every panels corner. But one actuator, which simultaneously controls the common corner of the adjacent panels, will have different adjustment values according to the plane fitting equation based on adjacent panels. In this paper, an adjustment value at the corner of the adjacent panel is obtained by plane fitting and adjustment calculation method with the antenna illumination function as the weight, that is, the optimal adjustment value of 1104 actuators of the Tianma 65 m radio telescope. Through many adjustments and the application of new algorithms, the surface accuracy of the reflector of the Tianma 65 m radio telescope has gradually improved to the current 0.24 mm.